Flow cytometric measurement of telomere length
نویسندگان
چکیده
منابع مشابه
Direct Comparison of Flow-FISH and qPCR as Diagnostic Tests for Telomere Length Measurement in Humans
Telomere length measurement is an essential test for the diagnosis of telomeropathies, which are caused by excessive telomere erosion. Commonly used methods are terminal restriction fragment (TRF) analysis by Southern blot, fluorescence in situ hybridization coupled with flow cytometry (flow-FISH), and quantitative PCR (qPCR). Although these methods have been used in the clinic, they have not b...
متن کاملThe flow cytometric analysis of telomere length in antigen-specific CD8+ T cells during acute Epstein-Barr virus infection.
Acute infectious mononucleosis (AIM) induced by Epstein-Barr virus (EBV) infection is characterized by extensive expansion of antigen-specific CD8+ T cells. One potential consequence of this considerable proliferative activity is telomere shortening, which predisposes the EBV-specific cells to replicative senescence. To investigate this, a method was developed that enables the simultaneous iden...
متن کاملThe flow cytometric analysis of telomere length in antigen-specific CD81 T cells during acute Epstein-Barr virus infection
http://bloodjournal.hematologylibrary.org/cgi/content/full/97/3/700 Updated information and services can be found at: (3443 articles) Immunobiology collections: Blood Articles on similar topics may be found in the following http://bloodjournal.hematologylibrary.org/misc/rights.dtl#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: ht...
متن کاملTelomere length measurement by fluorescence in situ hybridization and flow cytometry.
Telomere length is an important measure of cellular differentiation and progression to senescence. Flow cytometric assays for measuring telomere length have become an important adjunct to more laborious Southern blotting methods; telomere length can be estimated with considerable accuracy in small numbers of individual cells by flow cytometry, and can be measured in cell population subsets with...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry
سال: 2000
ISSN: 0196-4763,1097-0320
DOI: 10.1002/1097-0320(20000615)42:3<159::aid-cyto1>3.0.co;2-9